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KMID : 0613820110210010155
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Journal of Life Science
2011 Volume.21 No. 1 p.155 ~ p.158
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Association of a Single Nucleotide Polymorphism with Economic Traits in Porcine Uncoupling Protein 3 Gene
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Oh Jea-Don
Lee Kun-Woo
Jung Il-Jung
Jeon Gwang-Joo
Lee Hak-Kyo
Kong Hong-Sik
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Abstract
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Uncoupling protein (UCP) 3 has a number of proposed roles in the regulation of fatty acid metabolism. A number of polymorphisms in the human UCP3 gene have been identified, and the correlation with obesity related phenotypes evaluated. The objective of this study was to identify SNP in porcine UCP3 gene and to investigate the effect of the SNP on economic traits. The sequencing analysis method was used to identify nucleotide polymorphisms at position 1405 bp (Genebank accession No : AY739704) in porcine UCP3 gene. The SNP (G150R), located in the exon 3, changed the amino acid to glycine (GGG) from arginine (AGG). This G150R showed three genotypes - GG, GR and RR - by digestion with the restriction enzyme Sma Ⅰ using the PCR-RFLP method. The G150R showed significant effects only on back fat (P＜0.05). Animals with the genotype GG had significantly higher back fat thickness (1.358 ㎝) than animals with the genotype GR (1.288 ㎝, P＜0.05) and RR (1.286 ㎝, P＜0.05). However, the genotypes had no significant association with ADG and days to 90㎏. According to results of this study, a G allele of the G150R was found to have a significant effect on back fat thickness. It will be possible to use SNP markers on selected pigs to improve backfat thickness, an important economic trait.
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KEYWORD
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Uncoupling protein 3 (UCP3), SNP, backfat thickness, pig
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